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Josh and Rachel are in Florida looking into the case of the Daytona Beach Serial Killer. A chance encounter with the daughter of one of the victims propels them to investigate a string of leads including a former junkyard owner with a dark family secret and a notorious motorcycle gang with a motive to kill. Josh and Rachel discover an even greater horror, one that extends far beyond the beaches of Daytona and spreads through American highways.

After a private investigator's alarming revelation that there exists long haul truckers moonlighting as serial killers terrorizing America's interstate system, Josh and Rachel begin a cross-country journey to research these killers on wheels. While hitchhiking, the filmmakers encounter women who work truck stops, despairingly referred to as "lot lizards," and hear first hand accounts of the dangers they experience everyday. As Josh and Rachel delve deeper, they uncover systemic failures in law enforcement allowing these mobile killers to run wild. A disturbing confession by a former trucker, serving a life sentence for murder, propels Josh and Rachel on a hunt for his accomplices. When a potential serial killer turns up dead in West Virginia, the investigation takes a surprising turn and we learn that things are not always what they seem.

Discovering that the current systems in place to detect serial killers are severely broken, and suffering from an inability to connect the dots, Rachel and Josh head to New Mexico to experience firsthand just how bad things have become. After quickly being shut down by Albuquerque police, Rachel and Josh find themselves searching for a drifter who left a mysterious message for a local PI including details of the case. And soon they realize eerie connections between the victims expose an even darker reality, one they've yet to experience in their investigations so far.

With the help of local advocates and ex-FBI, Rachel and Josh search for eight missing women on Albuquerque's West Mesa when they discover they may be dealing with something even more sinister than a lone serial killer. In an attempt to uncover clues in New Mexico, Rachel and Josh score a lead from a self admitted serial killer that's too tempting to ignore which has them turning their lens back to Long Island and to a meeting with an unusual and dark individual. While tracking down this lead, a break in the Gilgo case exposes corruption and brings them back to the beginning and a new hope.

In the wake of Shannan Gilbert's funeral, family lawyer John Ray presents Josh and Rachel with confidential testimony that implicates a local doctor and Oak Beach resident in the death of the young escort. Yet, a critical questions remains unanswered, is Shannan's death connected to the four escorts discovered at Gilgo Beach? In order to find out, Josh and Rachel return to the secluded Oak Beach community where one family despite threats from several residents is willing to speak out. Meanwhile, rumors surface that the murders at Gilgo Beach are linked to a serial murder case in Atlantic City, which took place less than five years prior and offers eerie similarities. They decide to pay a visit to the City of Sin.

Josh and Rachel, continue their search for the Eastbound Strangler in Atlantic City. They revisit the motel where the bodies were dumped and tour the site with a man who at the time was the police's person of interest. A phone call with the family of one of the victims propels the film makers to find out why this case hasn't been solved, leading to an encounter with a sex worker, who believes she came face to face with the Eastbound Strangler. Eventually a call with an anonymous web sleuther sends Josh and Rachel to the site where the four women were possibly killed before they discover that the answer to this puzzle might lie outside Atlantic City.

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This episode features the rise and fall of Johnny Eng, one of the most notorious drug traffickers in the history of Manhattan's Chinatown. A one-time informant for the DEA, "Machine Gun Johnny" thought he could snitch on all his rivals to monopolize the heroin trade on the eastern seaboard. The special task force set up by the DEA would chase Eng all the way to Hong Kong. Eventually extradited to the U.S., Eng would hire John Gotti's lawyer to defend him against a prosecutor known as "The Dragon Lady."

In a decade long criminal run, the Cutt Boyz used violence to control the drug trade within the B.W. Cooper Housing Complex in New Orleans. Washington became the main supplier of Heroin, while Benjamin grew his murderous reputation as a main enforcer and was eventually responsible for three murders. Following a unique investigation approach, federal authorities indicted 11 Cutt Boyz under the RICO statutes for narcotics distribution and murder. But federal authorities weren't prepared for Hurricane Katrina's catastrophic destruction in 2005, which damaged evidence and displaced witnesses. Ultimately, they were able to piece together the case and successfully convicted all 11 Cutt Boyz. Washington received a 20-year sentence, while Benjamin will spend the rest of his life behind bars.

Moya and his chief enforcer, Andre Paige, led a deadly narcotics and extortion racket in New York City until the law caught up with them. With a failing heart, Moya escaped conviction and was sent home to die. Authorities would soon discover that he had received a new heart on the taxpayer's dime. With a new lease on life, Moya would disappear without a trace and stir up a manhunt beyond the jurisdiction of the United States.

This episode follows the case of Juan Raul Garza, the first drug kingpin. Garza and his gang made millions of dollars running drugs across "Marijuana Boulevard," the Gateway bridge linking Mexico and the U.S. in Brownsville, Texas. He became the first felon targeted for death by the federal government in more than 30 years.

Carlos Marcello grew up in the mysterious milieu of New Orleans in the early 20th Century to become the city's mafia boss for almost four decades. Despite his modest beginnings, Carlos joined the mafia at age 26 and quickly climbed in the ranks, aided by success in illegal gambling ventures and a talent for making effective payoffs to public officials. In 1947, Marcello became the longtime boss of New Orleans in a reign marked by absolute power, corruption, and antagonism of the U.S. government. At his height he would be revered as possibly the most powerful mob leader in the nation. One day Congress would even suspect Marcello of participating in a conspiracy to kill the President of the United States.

Made famous by Martin Scorcese's film Goodfellas, Jimmy "The Gent" Burke was one of the most prolific mob earners in New York history. But after the success of the infamous Lufthansa Heist, Burke unleashed a wave of murders that rocked New York City, and led to his downfall.

Fashioning himself a modern-day Celtic warrior, 1970's Irish mobster Danny Greene clashed with the Cleveland mafia, resulting in a bombing war that would eventually destroy them both.

After New York's Bonanno mob family was nearly destroyed by an undercover FBI agent, Joseph "Big Joey" Massino brought them back to prominence and became the only major Mafia boss left on the streets...the "Last Don."

For over thirty years, Gregory Scarpa lived a charmed triple life: mafia hit man, loving father and husband, and secret FBI informant--until a fatal disease weakened the man that had become known as "The Grim Reaper."

For over thirty years, Richard Kuklinski lived a shocking double life: suburban husband and father at home, and brutal contract killer on the streets--a job that give him the nickname "The Ice Man."

During the 1970s, Anthony "Gaspipe" Casso rose through the ranks of the New York Mafia to become underboss of the Lucchese family, raking in millions of dollars through elaborate scams and killing anyone who got in his way.

With over 30 years as the head of the Colombo crime family, Carmine Persico stands as one of most enduring Godfathers in mafia history--but his time at the top came from a ruthless drive for power, a drive that earned him the nickname, "The Snake."

Part 1 tells of the childhood and youth of John Gotti Junior. While growing up in Howard Beach, New York, and as a student at New York Military Academy, John learns of, and we follow the story of his father's meteoric rise to power in the world of organized crime. Years later, after graduating, instead of going on to pursue a career in the military, at his father's urging John Junior takes a year off. That's when his troubles begin and he gets in a barroom brawl. John's father "punishes" him by forcing him to spend his days hanging out at the Bergin Hunt and Fish Social Club, his father's mob headquarters. By the time John is in his twenties, he is asked and chooses to become a made member of the Gambino Crime Family.

The FBI is focused on taking down Gotti and the Gambino Crime family, as well as the bosses of all five New York mafia clans. When Gotti's underboss, Sammy Gravano agrees to testify for the government, Gotti Senior is convicted and sentenced to life in prison. John Junior becomes the acting boss of the crime family. With this transition of power, John Junior quickly becomes the prime target of the FBI. John Junior, having resolved to quit the life of organized crime, pleads guilty to racketeering, serves over seven years in prison, and must still withstand four federal trials all ending in mistrials before he is set free to pursue his life as a "civilian," a husband to his wife, father to his five children, and a law abiding citizen who honors his vow to quit the mob.